Standard Guide for Quantitating Cell Viability Within Biomaterial Scaffolds
1.1 This guide is a resource of cell viability test methods that can be used to assess the number and distribution of viable and non-viable cells within porous and non-porous, hard or soft biomaterial scaffolds, such as those used in tissue engineered medical products (TEMPs).
1.2 In addition to providing a compendium of available techniques, this guide describes materials specific interactions with the cell assays that can interfere with accurate cell viability analysis, and includes guidance on how to avoid, and/or account for, scaffold material/cell viability assay interactions.
1.3 These methods can be used for 3-D scaffolds containing cells that have been cultured in vitro or for scaffold/cell constructs that are retrieved after implantation in living organisms.
1.4 This guide does not propose acceptance criteria based on the application of cell viability test methods.
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
Please first log in with a verified email before subscribing to alerts.
Your Alert Profile lists the documents that will be monitored. If the document is revised or amended, you will be notified by email. You may delete a document from your Alert Profile at any time. To add a document to your Profile Alert, search for the document and click “alert me”.
Your Alert Profile lists the documents that will be monitored. If the document is revised or amended, you will be notified by email. You may delete a document from your Alert Profile at any time. To add a document to your Profile Alert, search for the document and click “alert me”.
Add to Alert
Already Subscribed to this document.
Your Alert Profile lists the documents that will be monitored. If the document is revised or amended, you will be notified by email. You may delete a document from your Alert Profile at any time. To add a document to your Profile Alert, search for the document and click “alert me”.
PDF Document format
Documents sold on the ANSI Webstore are in electronic Adobe Acrobat PDF format.
DRM protected documents
Some PDF files are protected by Digital Rights Management (DRM) at the request of the copyright holder. You can download and open this file to your own computer but DRM prevents opening this file on another computer, including a networked server. Some copyright holders may impose other restrictions that limit document printing and copy/paste of documents.
Non printable documents
Those documents cannot be printed at the request of the copyright holder.
We have no amendments or corrections for this standard.
Are the documents at the ANSI Webstore in electronic Adobe Acrobat PDF format only?
Documents sold on the ANSI Standards Store are in electronic Adobe Acrobat PDF format.
Standard Subscriptions
This standard is also available to be included in Standards Subscriptions. Standards Subscriptions from ANSI provides a money-saving, multi-user solution for accessing standards.
Subscription pricing is determined by: the specific standard(s) or collections of standards, the number of locations accessing the standards, and the number of employees that need access.
As the voice of the U.S. standards and conformity assessment system, the American National Standards Institute (ANSI) empowers its members and constituents to strengthen the U.S.
marketplace position in the global economy while helping to assure the safety and health of consumers and the protection of the environment.