Historical
I/LA24-A
Fluorescence Calibration and Quantitative Measurement of Fluorescence Intensity; Approved Guideline
Quantitative fluorescence calibration (QFC) is an empiric system to calibrate fluorescence intensity in a way that preserves stoichiometry between the concentration of fluorochrome in solutions and the equivalent molar quantity of fluorochrome on stained measurands such as cells, gels, microspheres, and microdots. This guideline describes the basic principles, reference materials, and laboratory procedures upon which QFC is based. This guideline is intended for use with reference materials and procedures developed under the National Institute of Standards and Technology (NIST) Fluorescence Intensity Standards program. While the general principles of QFC apply to any fluorescence measurement, this guideline specifically addresses analysis of cells and microspheres by flow cytometry, including cellular immunophenotyping and suspension array technology. The current and emerging uses of these laboratory methods will have an increasing impact on public health and primary care, from large-scale screening of populations to the individual profiling of each patient's disease.
Clinical And Laboratory Standards Institute [clsi]