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ASTM E2227-13

Standard Guide for Forensic Examination of Non-Reactive Dyes in Textile Fibers by Thin-Layer Chromatography

1.1 Metameric coloration of fibers can be detected using UV/visible spectrophotometry. If spectrophotometry is restricted to the visible spectral range only, differences in dye components may remain undetected. One method of detecting additional components is to use thin-layer chromatography (TLC). TLC is an inexpensive, simple, well-documented technique that, under certain conditions, can be used to complement the use of visible spectroscopy in comparisons of fiber colorants. The principle of the method is that the dye components are separated by their differential migration caused by a mobile phase flowing through a porous, adsorptive medium.

1.2 This standard does not replace knowledge, skill, ability, experience, education, or training and should be used in conjunction with professional judgment.

1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


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