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ANSI/ATCC ASN-0002-2021

Authentication Of Human Cell Lines: Standardization Of Short Tandem Repeat (STR) Profiling

This document elaborates a standardized procedure for unambiguous authentication and identification of human cell lines using Short Tandem Repeat (STR) profiling. The overall objective of this standard is to specify the methodology for STR profiling, data analyses, quality control of the data, interpretation of results, and implementation of a searchable public database.  The revision is drastically changed from the original version and expanded with many new sections explaining different aspects of STR profiling of human cell lines, including: guidelines for avoiding cell line cross-contaminations, explanations of the data and associated artifacts, descriptions of how cell lines can differ from each other due to being mixed cultures or manifesting microsatellite instability (MSI), and other types of results. The text is aimed at both the laboratory personnel who produce and process the data (personnel in core and commercial labs) and at the research laboratory personnel from PI to student who must interpret the results. The goals of this new revision are that all of these researchers understand their STR data, can interpret them correctly, and can identify when the results are not valid. This understanding is essential for the production of valid STR profiles for human cell lines so that we can avoid using misidentified cell lines in biomedical research.

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