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DIN EN ISO 14902:2022
Animal feeding stuffs - Determination of trypsin inhibitor activity of soya products (ISO 14902:2001); German version EN ISO 14902:2001
This International Standard specifies a method for the determination of the trypsin inhibitor activity (TIA) of soya products. This trypsin inhibitor activity is indicative of the degree of toasting of these products. The detection limit of the method is 0,5 mg/g. Trypsin inhibitors are extracted from the sample at pH 9,5. The remaining trypsin activity is measured by adding benzoyl-L-arginine-p-nitroanilide (L-BAPA) as substrate. The quantity of released p-nitroaniline is measured spectrometrically.
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Deutsches Institut für Normung [din]
Deutsches Institut für Normung [din]