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CLSI M06-A2

Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard - Second Edition


Clinical and Laboratory Standards Institute document M6-A2 - Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard-Second Edition describes three protocols for the evaluation of dehydrated Mueller-Hinton agar in the disk diffusion procedure for antimicrobial susceptibility testing. The first protocol is for use by manufacturers to evaluate production lots of Mueller-Hinton agar. The second and third are for selection and stability testing of primary and secondary reference lots of Mueller-Hinton agar. Performance of production lots is determined by comparison with the primary standard lot, which is provided to manufacturers upon request. The secondary standard lot may be used when the supply of the primary standard is threatened or its performance becomes unsatisfactory. Acceptable lots may be labeled with a statement indicating that the lot was tested according to the protocol found in this document and meets the acceptance limits of that protocol. These acceptance criteria apply only to disk diffusion procedures. They do not qualify Mueller-Hinton agar for other methods, such as agar dilution or antibiotic gradient.


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