Historical
CLSI M41-A
Viral Culture; Approved Guideline
This document provides guidance for viral culture and identification procedures that are typically performed in the clinical virology laboratory setting using commercially available cell cultures and reagents. The nature of the cell culture system is one that is inherently variable and thus remains susceptible to numerous adverse conditions that can lead to unreliable results. Several critical elements that must be addressed in devising a viral culture procedure are identified. These include: cell culture selection, assessment and maintenance; cell culture verification and quality control; culture medium preparation and quality control; specimen collection and preparation; isolate identification; and result reporting and interpretation. The intended audience includes laboratories performing either limited or comprehensive viral cultures as well as those that are considering introduction of viral culture. Regardless of the viral diagnostic testing algorithm utilized by a laboratory, the basic principles of viral culture are universal.
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Clinical And Laboratory Standards Institute [clsi]