Historical

CLSI MM09-A

Nucleic Acid Sequencing Methods in Diagnostic Laboratory Medicine; Approved Guideline

Sequencing methods for genotyping have moved from the research laboratory into the clinical laboratory. Sequencing is an assay format of choice for very high complexity genotyping, especially when hundreds or thousands of bases of genetic sequence are analyzed. NCCLS document MM9-A - Nucleic Acid Sequencing Methods in Diagnostic Laboratory Medicine; Approved Guideline addresses automated, PCR-based, dideoxy-terminator, and primer extension sequencing done on gel- or capillarybased sequencers. Topics covered include specimen collection and handling, isolation of nucleic acid, amplification and sequencing of nucleic acids, and interpretation and reporting results. Quality control/assessment considerations are addressed for each step of the process as appropriate.

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Clinical And Laboratory Standards Institute [clsi]

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