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MM16-P
Use of External RNA Controls in Gene Expression Assays; Proposed Guideline
Clinical and Laboratory Standards Institute (CLSI) document MM16-P - Use of External RNA Controls in Gene Expression Assays; Proposed Guideline provides protocols supporting the use of external RNA controls in microarray and QRT-PCR-based gene expression experiments. This guideline addresses important issues associated with the use of external RNA controls as a tool for verification of technical performance, and in support of the evaluation of qualitative results for a specific clinical analyte including preparation of control transcripts, design of primers and amplicons, quality control, use in final experimental or clinical test application, and the analysis and interpretation of data obtained. The guideline will facilitate research and clinical laboratories, regulatory agencies, accrediting agencies, reference laboratories, as well as test, microarray, and reagent manufacturers in measuring the performance of expression assays.
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Clinical And Laboratory Standards Institute [clsi]