DS/ISO 16140-3:2021/Amd 1:2025

Microbiology of the food chain - Method validation - Part 3: Protocol for the verification of reference methods and validated alternative methods in a single laboratory - Amendment 1: Protocol for verification of validated identification methods of microorganisms

This document specifies the protocol for the verification of reference methods and validated alternative methods for implementation in the user laboratory.*This document is applicable to the verification of methods used for the analysis (detection and/or quantification), confirmation and typing of microorganisms in:*– products intended for human consumption;*– products intended for animal feeding;*– environmental samples in the area of food and feed production, handling;*– samples from the primary production stage.*This document is, in particular, applicable to bacteria and fungi. Some clauses can be applicable to other (micro)organisms or their metabolites, to be determined on a case-by-case basis.*The technical protocols for the verification of validated qualitative methods and validated quantitative methods are described in Clauses 5 and 6. The technical protocol for the verification of validated alternative confirmation and typing methods is described in Clause 7. The protocols for the verification of non-validated reference methods are described in Annex F.