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ISO 21501-2:2007

Determination of particle size distribution - Single particle light interaction methods - Part 2: Light scattering liquid-borne particle counter

ISO 21501-2:2007 describes a calibration and verification method for a light scattering liquid-borne particle counter (LSLPC), which is used to measure the size and particle number concentration of particles suspended in liquid. The light scattering method described in ISO 21501-2:2007 is based on single particle measurements. The typical size range of particles measured by this method is between 0,1 µm and 10 µm in particle size.

Instruments that conform to ISO 21501-2:2007 are used for the evaluation of the cleanliness of pure water and chemicals, as well as the measurement of number and size distribution of particles in various liquids. The measured particle size using the LSLPC depends on the refractive index of particles and medium; therefore the measured particle size is equivalent to the calibration particles in pure water.

The following are within the scope of ISO 21501-2:2007:

  • size calibration;
  • verification of size setting;
  • counting efficiency;
  • size resolution;
  • false count rate;
  • maximum particle number concentration;
  • sampling flow rate;
  • sampling time;
  • sampling volume;
  • calibration interval;
  • test report.


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International Organization for Standardization [iso]


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