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Tests for Bacteria in Wastewater

Standards for testing wastewater for bacteria, toxicity, and biodegradable material specify test and screening methods. Detailing the scenarios in which a particular test is applicable, as well as the sampling and scale elements of a test, these standards address the detection and enumeration of multiple types of bacteria, as well as both the effects of wastewater on bacteria and the effects of the bacteria on larger forms of life.

ISO 10705-1:1995

Water quality -- Detection and enumeration of bacteriophages -- Part 1: Enumeration of F-specific RNA bacteriophages

Specifies a method for the detection and enumeration of F-specific ribonucleic acid bacteriophages. The sample is incubated with an appropriate host strain. Applicable to all kinds of water, sediments and sludges, even for shellfish extracts. The presence of F-specific RNA bacteriophages in a water sample indicates pollution by wastewater contaminated by human or animal faeces.

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ASTM D5392-19

Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure

1.1 This test method describes a membrane filter (MF) procedure for the detection and enumeration of Escherichia coli , a bacterium found exclusively in the feces of humans and other warm-blooded animals. The presence of these microorganisms in water is an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit of this procedure is one colony forming unit (CFU) per volume filtered. 1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user s responsibility to ensure the validity of this test method for waters of other types. 1.3 The values stated in SI units are to be regarded as standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9 . 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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ASTM D6503-19

Standard Test Method for Enterococci in Water Using Enterolert

1.1 This test method covers a simple procedure for the detection of enterococci in water and wastewater. It is based on IDEXX s patented Defined Substrate Technology (DST). 2 This product, Enterolert, utilizes a nutrient indicator that fluoresces when metabolized. It can detect these bacteria at one most probable number (MPN)/100 mL within 24 h. The presence of this microorganism in water is an indication of fecal contamination and the possible presence of enteric pathogens. 1.2 This test method can be used successfully with drinking water, source water, recreational (fresh and marine) water, wastewater, and bottled water. It is the user s responsibility to ensure the validity of this test method for waters of untested matrices. 1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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ASTM D5259-19

Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure

1.1 This test method covers a membrane filter (MF) procedure for the detection and enumeration of the enterococci bacteria in water. The enterococci, which include Entero-coccus faecalis ( E. faecalis ), E. faecium , and their varieties are commonly found in the feces of humans and other warm-blooded animals. Although some strains are ubiquitous and not related to fecal pollution, enterococci in water are an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit is one colony forming unit (CFU)/volume filtered. 1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user s responsibility to ensure the validity of this test method for waters of untested types. 1.3 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are included for information only and are not considered standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9 . 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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