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DIN EN ISO 19040-1:2023
Water quality - Determination of the estrogenic potential of water and waste water - Part 1: Yeast estrogen screen (Saccharomyces cerevisiae) (ISO 19040-1:2018); German version EN ISO 19040-1:2022
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: - fresh water; - waste water; - aqueous extracts and leachates; - eluates of sediments (fresh water); - pore water; - aqueous solutions of single substances or of chemical mixtures; - drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17?-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17?-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.
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Deutsches Institut für Normung [din]