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ISO 9439:1999

Water quality -- Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium -- Carbon dioxide evolution test

This International Standard specifies a method, by determination of carbon dioxide (CO2), for the evaluation in anaqueous medium of the ultimate biodegradability of organic compounds at a given concentration by aerobic microorganisms.

The method applies to organic compounds which are:

  • water-soluble under the conditions of the test, in which case removal of DOC may be determined as additional information (see annex D);
  • poorly water-soluble under the conditions of the test, in which case special measures may be necessary to achieve good dispersion of the compound (see, for example, ISO 10634);
  • non-volatile or which have a negligible vapour pressure under the conditions of the test;
  • not inhibitory to the test microorganisms at the concentration chosen for the test.


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